UC知道哪位大大给翻译一下！！
来源：百度知道 编辑：UC知道 时间：2024/05/09 12:08:14
Mass detection and MSn experiments were performed on a ThermoFinnigan Surveyor HPLC system (MS pump, PDA,autosampler) coupled to a ThermoFinnigan LCQ AdvantageMaxmass spectrometer (Dreieich, Germany) equipped with an ESI source and an ion trap mass analyzer. The whole system was controlled by Xcalibur software. For anthocyanins, the mass spectrometer was operated in the positive mode with the following conditions: source voltage 4.5 kV; capillary voltage 32 V; capillary temperature 275 ◦C; collision energy 30 (MS2) and 33% (MS3). Chromatographic separation was achieved on a 125 mm×2 mm i.d., 5 μm RP-Reprosil-Pur 120 ODS-3 column (Maisch,Ammerbuch, Germany) protected with a guard column of the same material in a cartridge holder. Sample preparation was done as described for colourless polyphenols. Injection volumewas 5μL, elution conditions were: 200μL/min flowrate at 25 ◦C; solventAwas 1% formic acid; solvent B,
Hplc PDA/electrospray 电离(花青素许多侦查ESI)-MS 和MSn 实验执行了在ThermoFinnigan 测量员HPLC 系统(女士泵浦, PDA, autosampler) 被结合对ThermoFinnigan LCQ AdvantageMaxmass 分光仪(Dreieich, 德国) 被装备以ESI 来源和离子陷井许多分析仪。整体系统由Xcalibur 软件控制了。为花青素, 质谱仪被管理在正面方式下以以下条件: 来源电压4.5 千伏; 血丝电压32 V; 血丝温度275?C; 碰撞能量30 (MS2) 并且33% (MS3) 。色谱分析的分离达到了在125 mm□毫米i.d., 5?m Rp Reprosil Pur 120 ODS-3 专栏(Maisch 、Ammerbuch, 德国) 被保护与同样材料的卫兵专栏在弹药筒囤户。样品准备完成如所描述为无色的多酚。射入volumewas 5?L, elution 情况是: 200?L/min 流速在25?C; solventAwas 1% 甲酸; 溶剂B, 50% 乙腈被调整的to1%formic 酸; 1min isocratic 条件与10% B, 线性梯度从10to 25% B 在24 分钟, 被跟随的bywashing 之内与100%Band reequilibrating 专栏。在第一7min 奔跑期间流出物被排泄了入废物在交换牵制阀门对离子来源避免女士的污秽用糖、有机酸, 和矿物从汁液之前。测量被执行了使用高峰地区(520 毫微米) 从外在定标通过参考物质cyanidin 3 苷(Extrasynthese 、Genay, 法国) 。一团线性定标曲线从5-50 mg/L 被使用了。所有HPLC 奔跑完成在duplicate.The 果子里从不同的品种并且被分成了入皮肤和骨肉被结冰干燥跟随。被冰冻干燥的和被研的材料的一百毫克被提取了以5 机器语言酸化的甲醇(0.1% HCl).The 萃取物是0.45?m 被过滤在HPLC 射入之前。