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来源：百度知道 编辑：UC知道 时间：2024/05/05 19:17:19
MATERIALS AND METHODS: The material for this investigation of expression of mRNA selected genes was 4 tissue specimens from women with vulvar cancer T1N2M0. The method used for quantitative determination of the numbers of mRNA copies of selected genes was the QRT-PCR and QPCR technique, with the use of an ABI PRISM 7700 (TaqMan) sequence detector. The nucleotide sequence for starters and probes for quantitative RT-PCR was designed with the use of the Primer Express Version 1.0 ABI PRISM software.
RESULTS: Expression of all mRNA isoforms VEGF was found in the samples. The number of mRNA copies obtained in 1 microgram total mRNA extract of the genes examined in tissue sections with various pathomorphology were clearly differentiated. CONCLUSIONS: Expression profile of aforementioned genes indicated a definitive relationship between vascular endothelial growth factor isoform and vulvar cancer. These findings provide evidence that the expression pattern of mRNA VEGF isoform should fi
RESULTS: Expression of all mRNA isoforms VEGF was found in the samples. The number of mRNA copies obtained in 1 microgram total mRNA extract of the genes examined in tissue sections with various pathomorphology were clearly differentiated. CONCLUSIONS: Expression profile of aforementioned genes indicated a definitive relationship between vascular endothelial growth factor isoform and vulvar cancer. These findings provide evidence that the expression pattern of mRNA VEGF isoform should fi
材料和方法：这次用来做研究的被挑选的mRNA基因的材料是4个从患有外阴癌T1N2M0的妇女中取得的样本组织。这种用来定量测定被挑选基因的mRNA副本的数目的方法是QRT - PCR和QPCR技术，借助了一种称之为ABI PRISM 7700 ( TaqMan)的序列检测器。通过利用Primer Express 1.0版本ABI软件来设计定量的RT-PCR技术的起动器和探针获得的核苷酸序列。
结果：在所有mRNA当中对碘氧基苯甲醚VEGF的表达式在样品中均有发现。切片组织经各种的病理形态学考核后，在其基因中总计1微克的mRNA萃取物中获得的mRNA副本数目被清晰地区别开来。
结论：上述基因分布表达式表明了在血管的内皮的增长因子和外阴癌之间的一种确定的关系。这项发现为mRNA VEGF对碘氧基苯甲醚的表达式图象将在临床的和研究方向得到广泛的应用提供了证据。