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来源：百度知道 编辑：UC知道 时间：2024/05/22 12:09:10
摘要：目的：建立槐米中芦丁含量测定的高效液相色谱法。方法：以岛津ODS色谱柱(5 μm, 150 mm× 4.6 mm )为固定相，以甲醇—1%乙酸（40：60）为流动相，柱温35。C， 检测波长257nm；流速为1.0ml/min。结果：芦丁与槐米中其他成分基线分离良好，在0.024－0.056mg/ml的范围内进样浓度与其色谱峰面积值呈良好的线性关系，回归方程为：Y=3E+07X－94270，R=0.9990，平均回收率为98.44% 。结论：该方法处理简单，准确度高，分离效能高，适合于槐米中芦丁的含量测定。
关键词：槐米；芦丁；高效液相色谱法；含量测定

Abstract : Objective : To establish Huaimi Rutin Determination by HPLC. Methods : Shimadzu ODS column (5 μ m, 150 mm × 4.6 mm) as the stationary phase, methanol-1% acetic acid (40:60) as the mobile phase was 35. C, the detection wavelength 257nm; flow rate of 1.0ml/min. Results : Huaimi rutin and other components separated from the baseline, 0.024-0.056mg/ml in the context of influent concentration area with peaks value was linear relations Department of regression equation : Y=3E+07X-94270, 0.9990, The average recovery of 98.44%. Conclusion : The method is simple with high accuracy and high separation efficiency, suitable for Huaimi Rutin Determination. Keywords : Huaimi; Rutin; HPLC; Determination