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Transposable elements are regarded as a powerful
mutagen and as an effective tool to isolate genes tagged
by transposon insertions. The Japanese morning glory
contains around 500-1000 copies of an En/Spm-related
element Tpn1 and its relatives, which act as major
spontaneous mutagens. We have previously developed
an amplified restriction fragment length polymorphism
(AFLP)-based mRNA fingerprinting (AMF) procedure
which is based on the systematic comparison of differently
expressed transcripts in the same tissue in different
lines, and succeeded in applying AMF for the identification
of a new mutable allele caused by integration
of a transposable element into an anthocyanin biosynthesis
gene. Since transposon mutagenesis has become a
powerful tool for the isolation of genes of interest, we
have attempted to develop a new protocol for identifying
tagged genes by insertion of Tpn1-related elements
in the

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