请高手帮忙翻译论文摘要。急!!!

来源:百度知道 编辑:UC知道 时间:2024/05/17 05:50:21
摘要:采用水稻成熟胚诱导愈伤组织,优化培养条件和培养时间,建立适合水稻成熟胚的遗传转化体系,从而为水稻建立高效的转基因体系提供技术基础。试验结果表明:外植体消毒 采用75%酒精和1‰的升汞处理外植体,升汞处理时间以15 min灭菌效果最好,诱导培养基只加2,4-D 2mg/L,分化培养基激素浓度应为6-BA 2mg/L+NAA 0。2mg/L,继代次数以2~3次为最佳。生根培养基适宜采用1/2倍浓度的MS培养基。

Make a summary: Ripen with paddy Pei guide recovers to injure organization , optimize training condition and training time, establishment suitable paddy maturation Pei hereditary transformation system, so for paddy, establishing efficient genetically engineered system offer technical foundation. Testing result shows: Outside plant body disinfection to adopt the 1 and alcohol of 75% ‰ litre mercury handling outside plant body, litre mercury handling time with the sterilization effect of 15min is best, guide culture medium to add 2 and 4-D2mg/L only, break up the hormone density of culture medium to should be 6-BA2mg/L+NAA0. 2mg/L, then acting frequency with 2 ~ three times are best. Take root MS of culture medium that suits to adopt the time density of 1/2 culture medium.