UC知道英文文献翻译（英译汉）
来源：百度知道 编辑：UC知道 时间：2024/06/07 01:31:21
Secondary structure in RNA targets is a significant barrier to short DNA probes. However, when such targets are the end product of an in vitro amplification scheme, it is possible to carry out transcription in the presence of nucleoside triphosphate analogues that reduce secondary structure of the RNA without impairing subsequent hybridization. Here we show that nucleoside triphosphates of 2-aminoadenine (nA) and 2-thiouracil (sU) are taken up by T7 RNA polymerase and that the resulting RNA possesses reduced secondary structure and improved accessibility to DNA probes. The hybridization properties of short RNA transcripts were studied using a new gel mobility shift assay from which melting temperatures were determined. RNA hairpins that contained nA and sU were able to hybridize to DNA probes under conditions where the unmodified hairpins did not. DNA-RNA hybrids that contained nA and sU in the RNA strand exhibited enhanced specifi
Secondary structure in RNA targets is a significant barrier to short DNA probes. However, when such targets are the end product of an in vitro amplification scheme, it is possible to carry out transcription in the presence of nucleoside triphosphate analogues that reduce secondary structure of the RNA without impairing subsequent hybridization.
目标RNA的二级结构对短DNA探针来说是一个严重的障碍，可是，如果这些靶RNA是人工体外增殖的产物，并且在三磷酸核苷存在的环境下，改变RNA的二级结构，DNA可以进行转录，并且不会影响杂交产物的特性
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二级结构的RNA的目标，是一个重大的障碍，以短DNA探针。但是，当这些指标是最终产品的一种体外扩增计划，是有可能进行转录在场的三磷酸核苷类似物减少二级结构的RNA上损害以后的杂交。在这里，我们证明了核苷triphosphates two aminoadenine 和 2 - thiouracil ，是由T7 RNA聚合酶，并由此产生的RNA具有降低二级结构和改善无障碍环境，以DNA探针。杂交性质的短期RNA转录进行了研究采用一种新的凝胶迁移率变动分析，从其中熔化温度进行了测定。 RNA的发夹含有钠，苏能杂交，以DNA探针的条件下，凡未经修改的发夹弯，没有信心。与DNA核酸杂交种含有钠，苏中的RNA链呈现增强的特殊性，增加了稳定性和更大的平等的基础配对强度比上年杂种未经修改。替代鸟嘌呤（ g ）和肌苷（一）进一步减少二级结构，RNA的这个基地杂交非选择性 。部队高度稳定和钠- T和一个素个碱基对的DNA核酸杂交种，再加上不稳定的影响，钠苏夫妇在RNA的目标，占改良杂交性能。这些结果表明团娜和苏期间，在体外转录是一种很有前途的策略和提高效能低聚DNA探针与RNA的目标。
在核糖核酸目标的二级