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来源：百度知道 编辑：UC知道 时间：2024/06/05 11:30:12
Measurements were started after steady-state
conditions were reached. Maximal fluorescence variations (Fmax%)represent the maximal observed percentage difference with respect to the fluorescence intensity at themoment of the solution substitution (iodide/chloride). Because iodide is able to quench the EYFP signal more efficiently than chloride (33), the
slight increase of fluorescence observed in control cells not over-expressing SLC26A4 (see Fig. 2, empty), is pointing to the fact that the chloride exit prevails the iodide influx. Therefore, the transporters or channels able to pass iodide from the extracellular space toward the cytosol are scarcely active in the
native HEK 293 Phoenix cell line we use. This cell line is therefore particularly suited to test SLC26A4-induced halide transport activity after the overexpression
of the transporter or its mutants. After the expression of WT
SLC26A4, however, the exchange of extracellular chloride with iodide leads
conditions were reached. Maximal fluorescence variations (Fmax%)represent the maximal observed percentage difference with respect to the fluorescence intensity at themoment of the solution substitution (iodide/chloride). Because iodide is able to quench the EYFP signal more efficiently than chloride (33), the
slight increase of fluorescence observed in control cells not over-expressing SLC26A4 (see Fig. 2, empty), is pointing to the fact that the chloride exit prevails the iodide influx. Therefore, the transporters or channels able to pass iodide from the extracellular space toward the cytosol are scarcely active in the
native HEK 293 Phoenix cell line we use. This cell line is therefore particularly suited to test SLC26A4-induced halide transport activity after the overexpression
of the transporter or its mutants. After the expression of WT
SLC26A4, however, the exchange of extracellular chloride with iodide leads
后开始测量稳态
条件达成协议。最大荧光变化（的Fmax％）代表就在观察荧光强度最大百分比解决方案的替代themoment差异（碘/氯）。由于碘能止息EYFP信号进行更为有效的氯（33），在
荧光略有增加的现象，并非对控制细胞表达SLC26A4（见图。2，空），是指向一个事实，即氯化碘出口普遍存在的涌入。因此，运输或渠道能通过从胞浆细胞对碘的空间几乎活跃在
凤凰引领本土293细胞系，我们的使用。该细胞系，因此特别适合于测试SLC26A4诱导表达后，运输活动卤化物
该转运或其突变体。之后小波表达
SLC26A4，但是，与氯化碘外交换导致的，因为SLC26A4 EYFP荧光明显减少驱动碘涌入銆